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The Influence of Different Types of Antibodies on In Vitro Fertilization Results
Author(s) -
MARDESIC T.,
HUTTELOVA R.,
MULLER P.,
VOBORIL J.,
MIKOVA M.,
HULVERT J.,
ULCOVAGALLOVA Z.
Publication year - 2000
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.8755-8920.2000.430101.x
Subject(s) - andrology , in vitro fertilisation , intracytoplasmic sperm injection , human fertilization , infertility , antibody , hemagglutination , medicine , direct agglutination test , embryo transfer , hemagglutination assay , sperm , unexplained infertility , pregnancy , chemistry , gynecology , immunology , titer , biology , serology , genetics , anatomy
PROBLEM: The influence of anti‐sperm (ASA), anti‐phospholipid (APA), and anti‐zonal (AZA) antibodies on in vitro fertilization (IVF) results and the need for intracytoplasmic sperm injection (ICSI) were assessed. METHOD OF STUDY: Forty‐four couples with infertility of immunologic origin were investigated. ASA in serum and ovulatory mucus were studied by a tray agglutination test (TAT) and indirect mixed anti‐globulin reaction test (MAR) test, AZA were studied by passive hemagglutination and commercial enzyme‐linked immunosorbent assay (ELISA; BioGen, Germany), and APA were tested by ELISAs in immunoglobulin isotypes IgG and IgM. RESULTS: Because of failed or very low fertilization after standard IVF in the previous cycle, ICSI had to be used in five out of 15 cases with ASA (33.3%), in 16 out of 18 couples with AZA (89.4%), and in only one case if APA were present (9%). Clinical pregnancy rate was 60% in cases with ASA, 38.5% with AZA, and 27.3% per embryo transfer (ET) if APA were detected. CONCLUSIONS: Immunologic infertility can be treated by IVF with very good results. The most important group are women with AZA, in whom IVF–ICSI without any delay is recommended.