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Analysis of testicular‐internal spermatic vein variation and the recreation of varicocoele in a Sprague‐Dawley rat model
Author(s) -
Zhang L. T.,
Kim H. K.,
Choi B. R.,
Zhao C.,
Lee S. W.,
Jang K. Y.,
Park J. K.
Publication year - 2014
Publication title -
andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 2047-2927
pISSN - 2047-2919
DOI - 10.1111/j.2047-2927.2014.00201.x
Subject(s) - ligation , varicocele , spermatic vein , predictive value , andrology , left renal vein , urology , medicine , gynecology , biology , surgery , infertility , inferior vena cava , pregnancy , genetics
Summary Many laboratories tried to recreate the varicocoele model have met with varied success. To recreate a consistent varicocoele model by exploring the anatomic variability of the testicular‐spermatic venous system in Sprague‐Dawley ( SD ) rats. Seventy‐two sexually mature SD male rats were randomly divided into three groups containing 24 rats per group. Partial ligation of the left renal vein and internal spermatic vein ( ISV ) communicating branches to common iliac vein and ISV communicating branches ligation ( RVISVCBCIV ) or partial ligation of the left renal vein and ISV communicating branches ligation ( RVISVCB ). The results showed that the mean diameter of the left ISV was significantly increased in the RVISVCBCIV group compared with the control and RVISVCB groups ( p < 0.001). Using ISV as the reference, the sensitivity of varicocoele was 71.43%, and the specificity was 80%. In addition, the positive predictive value was 83.33%, and the negative predictive value was 66.67%. Sperm count, motility, Johnsen score and the spermatogenic cell density were lower in the RVISVCBCIV group compared with the control ( p < 0.01). The apoptotic index was higher in the RVISVCBCIV group compared with control groups ( p < 0.01). The RVISVCBCIV provides a more effective method for establishing a varicocoele‐induced model.