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Equine colostral carbohydrates reduce lipopolysaccharide‐induced inflammatory responses in equine peripheral blood mononuclear cells
Author(s) -
Vendrig J. C.,
Coffeng L. E.,
FinkGremmels J.
Publication year - 2012
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.2012.00680.x
Subject(s) - colostrum , peripheral blood mononuclear cell , lipopolysaccharide , tumor necrosis factor alpha , interleukin , immunology , proinflammatory cytokine , chemistry , inflammation , in vitro , cytokine , medicine , antibody , biochemistry
Summary Reasons for performing study Increasing evidence suggests that reactions to lipopolysaccharide ( LPS ), particularly in the gut, can be partly or completely mitigated by colostrum‐ and milk‐derived oligosaccharides. Confirmation of this hypothesis could lead to the development of new therapeutic concepts. Objectives To demonstrate the influence of equine colostral carbohydrates on the inflammatory response in an in vitro model with equine peripheral blood mononuclear cells ( PBMCs ). Methods Carbohydrates were extracted from mare colostrum, and then evaluated for their influence on LPS ‐induced inflammatory responses in PBMCs isolated from the same mares. mRNA expression of tumour necrosis factor‐α, interleukin‐6 and interleukin‐10 was measured as well as the protein levels of tumour necrosis factor‐α (TNF‐α) and interleukin‐10 (IL‐10). Results Equine colostral carbohydrates significantly reduced LPS ‐induced TNF ‐α protein at both times measured and significantly reduced LPS ‐induced TNF ‐α, IL ‐6 and IL ‐10 mRNA expression by PBMCs . Moreover, cell viability significantly increased in the presence of high concentrations of colostral carbohydrates. Conclusions Carbohydrates derived from equine colostrum reduce LPS ‐induced inflammatory responses of equine PBMCs . Potential relevance Colostrum and milk‐derived carbohydrates are promising candidates for new concepts in preventive and regenerative medicine.