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An explant based‐method for differentiating adipocytes from equine adipose tissue
Author(s) -
SUAGEE J. K.,
CORL B. A.
Publication year - 2013
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.2012.00581.x
Subject(s) - adipose tissue , explant culture , adipocyte , stromal cell , stromal vascular fraction , adipose tissue macrophages , biology , in vitro , chemistry , microbiology and biotechnology , medicine , endocrinology , white adipose tissue , biochemistry
Summary Culturing adipocytes enables fine control of experimental conditions and helps minimise animal use. This report describes an explant‐based method for isolating stromal‐vascular cells from equine adipose tissue that enables use of small amounts of tissue. Subcutaneous and mesenteric adipose tissues were harvested post mortem and stromal‐vascular cells grown from explants, prior to testing the capacity of several differentiation media to induce lipid droplet formation and increase transcript abundance of adipocyte markers. Inclusion of rosiglitazone at 1 and 5 µmol/l concentrations, along with other media components, induced differentiation of cultured equine stromal‐vascular cells derived from subcutaneous and mesenteric adipose tissues.