Premium
Induction of heat shock protein 72 mRNA in skeletal muscle by exercise and training
Author(s) -
PÖSÖ A. R.,
EKLUNDUUSITALO S.,
HYYPPÄ S.,
PIRILÄ E.
Publication year - 2002
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.2002.tb05421.x
Subject(s) - heat shock protein , skeletal muscle , messenger rna , hsp70 , treadmill , medicine , endocrinology , muscle biopsy , biology , biopsy , biochemistry , gene
Summary In response to stress, cells synthesise heat shock proteins (HSP) to maintain protein homeostasis. To study whether exercise and training induce expression of HSP72 in the middle gluteal muscle, 10 Finnhorses performed a submaximal 60 min exercise test on a treadmill. Test A was performed after 3 months of training, and the other two tests 2 (B) and 5 (C) weeks later. Blood samples were taken during and after the tests, and biopsy samples before, immediately after and 23 h after each test. HSP72 mRNA was analysed using a digoxigenin‐labelled probe. Blood lactate concentration in the 3 tests varied between 7.2 and 10.2 mmol/I. Training increased HSP72 mRNA, as indicated by increases in samples taken at rest (A