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Comparison of different methods for the recovery of horse oocytes
Author(s) -
ALM H.,
TORNER H.,
KANITZ W.,
BECKER F.,
HINRICHS K.
Publication year - 1997
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1997.tb05099.x
Subject(s) - follicle , oocyte , ovarian follicle , in vivo , folliculogenesis , biology , andrology , horse , ovary , endocrinology , medicine , microbiology and biotechnology , embryo , embryogenesis , genetics , paleontology
Summary The object of this study was to compare 4 different methods of oocyte recovery from mares; 1) transvaginal follicle aspiration in vivo ; 2) follicle aspiration in vitro ; 3) oocyte recovery by isolation of follicles in vitro and 4) follicle scraping in vitro. Oocyte recovery was highest after follicle scraping (71.1%) and follicle isolation and rupture (61.3%). Follicle aspiration in vitro and in vivo yielded oocytes on 31.2% and 19.3% of occasions, respectively. The output of different types of cumulus‐oocyte‐complexes was different among the methods; the portion of compact cumulus‐oocyte‐complexes was significantly higher with follicle scraping (50.7%) and follicle isolation (44.5%) than with aspiration in vivo (31.9%) and in vitro (23.7%). The recovery rate of oocytes from small follicles (<15 mm) was significantly higher than from larger follicles (P < 0.05) using transvaginal follicle aspiration. The proportion of oocytes that were degenerate (exhibited shrunken, dense or visibly damaged ooplasm) ranged from 1.2% after follicle scraping, to 17.2% after aspiration in vivo. These results indicate that, for the recovery of horse oocytes in vitro , follicle scraping and follicle isolation give the highest recoveries of cumulus‐intact oocytes.