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Cellular heterogeneity in cathepsin D distribution in equine articular cartilage
Author(s) -
HERNANDEZVIDAL G.,
JEFFCOTT L. B.,
DAVIES M. E.
Publication year - 1997
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1997.tb03122.x
Subject(s) - cathepsin , cartilage , cathepsin k , cathepsin d , anatomy , cathepsin l , horse , antiserum , pathology , chemistry , biology , microbiology and biotechnology , enzyme , medicine , immunology , antibody , biochemistry , in vitro , paleontology , osteoclast
Summary The distribution of cathepsin D in normal equine growth cartilage has been examined immunocytochemically using an antiserum raised against human cathepsin D. The cross‐reactivity and specificity of the antiserum for equine cathepsin D was confirmed, and its lysosomal localisation was demonstrated in horse skin fibroblasts by confocal scanning microscopy. Cultured horse chondrocytes were heterogenous in their expression of cathepsin D. Heterogeneity of distribution of the enzyme was also seen in chondrocytes in cartilage from different anatomical sites. A high level of cathepsin D was observed in the deep layer of cartilage from the lateral trochlear ridge of the distal femur. Cathepsin D was absent in the hypertrophic zone of the distal radial growth plate.