Premium
Age related changes in biochemical markers of bone metabolism in horses
Author(s) -
PRICE JOANNA S.,
JACKSON B.,
EASTELL R.,
GOODSHIP A. E.,
BLUMSOHN A.,
WRIGHT I.,
STONEHAM SARAH,
LANYON L. E.,
RUSSELL R. G. G.
Publication year - 1995
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1995.tb03063.x
Subject(s) - bone remodeling , physiology , medicine , biology
Summary Biochemical markers of bone metabolism were analysed in serum samples obtained from 60 horses with no history of orthopaedic disease (age 3 months‐20 years). Serum levels of the carboxyterminal propeptide of type I procollagen (PICP), a marker of bone formation and the pyridinoline cross linked telopeptide domain of type I collagen (ICTP), a putative marker of bone resorption, were measured by radioimmunoassay (RIA). Serum levels of the bone specific isoenzyme of alkaline phosphatase (BALP), another marker of bone formation, were measured by a wheatgerm agglutinin affinity (WGA) method. Total alkaline phosphatase levels were also determined. Serum levels of PICP were significantly correlated with bone ALP (r=0.78, P<0.0001) and ICTP (r=0.87, P<0.0001). ICTP levels also correlated significantly with bone ALP (r=0.81, P<0.0001). However, total alkaline phosphatase did not correlate significantly with PICP, ICTP and BALP in horses over 1 year of age. There was an inverse correlation between serum levels of all biochemical markers and age of animals, with the most significant changes seen over the first 2 years. In animals less than 1 year of age, the reference ranges (mean ± s.d. 1.96) were as follows: PICP 1216–2666 μg/l, ICTP 13.8–26.7 μg/l, bone ALP 134–288 u/l and total ALP 223–498 u/l. In 2‐year‐olds, the equivalent reference ranges were: PICP 550–1472 μg/l, ICTP 7.96‐22.8 μg/l, bone ALP 32.7–125 u/l and total ALP 134–238 u/l. Assays for bone ALP and antigens that reflect collagen metabolism display significant potential as sensitive non invasive methods of evaluating bone metabolism in the horse.