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Haematological, bone marrow and clinical chemical changes in neonatal foals given canine recombinant granulocyte‐colony stimulating factor
Author(s) -
ZINKL J. G.,
MADIGAN J. E.,
FRIDMANN D. M.,
KABBUR M. B.,
REYNALO'CONNOR J.,
ANDRESEN J. W.
Publication year - 1994
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1994.tb04392.x
Subject(s) - granulocyte colony stimulating factor , horse , granulocyte , medicine , bone marrow , hematology , alkaline phosphatase , endocrinology , andrology , biology , enzyme , biochemistry , chemotherapy , paleontology
Summary Five Thoroughbred and Quarter Horse cross foals were given 20 μg canine recombinant granulocyte‐colony stimulating factor (rcG‐CSF) per kg bwt intramuscularly (i.m.) on the day of birth and 10 μg rcG‐CSF/kg for 13 additional days. During this time and for an additional 21 days haematology, bone marrow and clinical chemical analyses were performed. After one day of rcG‐CSF administration leucocyte and neutrophil counts increased from 9.16 × 10 9 /1 to 23.44 × 10 9 /1 and from 6.45 × 10 9 /1 to 19.61 × 10 9 /1, respectively. The counts continued to increase for the next 3–4 days and then there was a slight decrease. A second increase followed and the leucocyte and neutrophil counts increased to 52.84 × 10 9 /1 and 45.16 × 10 9 /1 on the day after the last rcG‐CSF administration (Day 15). The counts decreased rapidly immediately after the administration of rcG‐CSF was stopped and then at a slower rate. The cell counts were still higher than in the controls at the end of the study period (Day 35). Bone marrow cellularity increased from 10–25% before rcG‐CSF was given to 60–80% after 5 days. The increase in cellularity was due to increased myeloid activity because the myeloid to erythroid ratio increased from 2.7 to 8.8. Serum chemistry changes were minimal although foals given rcG‐CSF at various times had lower glucose concentrations and increased alkaline phosphatase and gamma glutamyl transferase activities.