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Proteolytic enzymes in equine joints with infectious arthritis
Author(s) -
SPIERS SUE,
MAY S. A.,
HARRISON LUISE J.,
BENNETT D.,
EDWARDS G. B.
Publication year - 1994
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1994.tb04330.x
Subject(s) - synovial fluid , collagenase , arthritis , enzyme , synovial membrane , horse , medicine , immunology , proteolytic enzymes , infectious arthritis , microbiology and biotechnology , pathology , chemistry , biology , osteoarthritis , biochemistry , alternative medicine , paleontology
Summary Significant amounts of collagenase and caseinase activity were detected in infected synovial fluid samples. Partial characterisation of the enzymes by gel filtration suggested that synovial fluid from cases of infectious arthritis may contain enzymes from both the synovial cells and neutrophils. This finding was also supported by analysis of sequential synovial fluid samples from 4 infected joints. In 3 joints the concentration of caseinase and in 1 joint collagenase paralleled the decline in total nucleated cell count. However, in 3 joints the concentration of collagenase remained high after the total nucleated cell count had returned to normal, suggesting that this enzyme originated from resident articular cells.