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Preliminary results of demineralised bone matrix implantation in horses
Author(s) -
Douglas Janet,
Clarke A. F.
Publication year - 1993
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1993.tb04860.x
Subject(s) - endochondral ossification , cartilage , ossification , medicine , intramembranous ossification , bone matrix , hyaline cartilage , matrix (chemical analysis) , mesenchymal stem cell , surgery , anatomy , articular cartilage , pathology , chemistry , osteoarthritis , alternative medicine , chromatography
Summary In rats the subcutaneous or intramuscular implantation of allogeneic demineralised bone matrix (DBM) induces host mesenchymal cells to undergo endochondral ossification. In normal rats this process adheres to a rigid timetable, cartilage being present by Day 7 and bone by Day 12. This technique was applied to horses. Demineralised bone matrix from 3 different equine donors, a neonate, a yearling and a 4‐year old, was implanted intramuscularly or subcutaneously in 4 different equine hosts, aged 6 weeks to 6 years. Implants were removed 10–37 days later. No evidence of endochondral ossification was detected in any of the implants. As a control experiment, DBM prepared from rats was implanted into allogeneic hosts using the same bone preparation and implantation technique as was used in the horses. Endochondral ossification was induced in the rats, and the process followed the expected time course. Preliminary results suggest that hyaline cartilage formation does not occur in horses within 37 days. The present study does not exclude the possibility that endochondral ossification may occur in horses if longer implantation periods or different methods of DBM preparation are used.