Premium
Modifications in the technique used to recover equine embryos
Author(s) -
ALVARENGA M. A.,
ALVARENGA F. C. LANDIM,
MEIRA C.
Publication year - 1993
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1993.tb04841.x
Subject(s) - embryo , saline , uterus , significant difference , andrology , embryo transfer , phosphate buffered saline , pregnancy , recovery rate , pregnancy rate , horse , chemistry , gynecology , medicine , biology , anesthesia , chromatography , paleontology , genetics , microbiology and biotechnology
Summary In Experiment I, phosphate‐buffered saline (PBS) or Ringer‐lactate solution (RL) was used to flush embryos from mares, but there was no difference (P>0.05) in pregnancy rate after transfer of embryos: 8/14 (57%) in PBS and 14/22 in RL. There was also no difference (P>0.05) in pregnancy rates in Experiment II when embryos were flushed with PBS alone (53/117, 45%) or PBS + 1% foetal calf serum (37/70, 53%). In Experiment III, the standard technique for embryo recovery gave 55% (11/20) success compared with 75% (18/24) for a modified method whereby 3 min' contact time was allowed in the uterus for each flush, without massage of the uterus, but this was not statistically significant (P>0.05).