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Atlas of chromatin configurations of germinal vesicle‐stage and maturing horse oocytes
Author(s) -
HINRICHS K.,
SCHMIDT A. L.,
SELGRATH J. P.
Publication year - 1993
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1993.tb04828.x
Subject(s) - chromatin , germinal vesicle , nucleus , biology , chromatin remodeling , microbiology and biotechnology , oocyte , biophysics , chemistry , dna , genetics , embryo
Summary The fluorescent, DNA‐specific label, Hoechst 33258, was used to determine the chromatin configuration of immature and maturing horse oocytes. The stain was simple to use and effective in revealing the chromatin of horse oocytes without need for clearing of lipid. The chromatin configuration of oocytes known to have a germinal vesicle fell into two major categories: fluorescent nucleus, in which the entire nucleus fluoresced with or without clumps of chromatin within; and compact chromatin, in which only one compact mass of chromatin was visible. These classifications were further broken up into diffuse, spotty or crinkled fluorescing nucleus, and compact, faded or loosely compact condensed chromatin. The physiological relevance of the different chromatin configurations of germinal vesicle stage oocytes is presently unknown. The chromatin configurations of maturing oocytes were similar to those seen in other species.