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Diagnosis of Equid herpesviruses −1 and −4 by polymerase chain reaction
Author(s) -
SHARMA P. C.,
CULLINANE A. A.,
ONIONS D. E.,
NICOLSON L.
Publication year - 1992
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1992.tb02772.x
Subject(s) - polymerase chain reaction , virology , medicine , biology , genetics , gene
Summary The polymerase chain reaction (PCR) is a sensitive technique used to detect DNA of viral pathogens. We have applied the technique to the detection of Equid herpesviruses‐1 and −4 (EHV‐1 and EHV‐4) DNA within nasopharyngeal swab samples from horses. Ninety‐eight samples from suspected field cases and in‐contact horses were analysed. The assays were conducted blind and later decoded and compared with virus isolation data. Our results indicate that PCR is a sensitive and rapid technique for the diagnosis of EHV‐1 and EHV‐4 infection.