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Carbonic anhydrase III in equine tissues and sera determined by a highly sensitive enzyme‐immunoassay
Author(s) -
NISHITA T.,
MATSUSHITA H.
Publication year - 1990
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1990.tb04262.x
Subject(s) - immunoassay , carbonic anhydrase , enzyme , chemistry , horse , microbiology and biotechnology , biochemistry , carbonic anhydrase ii , biology , antibody , immunology , paleontology
Summary A sensitive sandwich enzyme immunoassay (EIA) for measuring equine carbonic anhydrase III (CA‐III) was established using a microplate as a solid‐phase and peroxidase as a labelling enzyme. The assay can detect concentrations as low as 5 ng/ml using 20 μl of sample sera. Within‐run coefficients of variation obtained using standard equine CA‐III were less than 5 per cent. CA‐III levels in equine serum ranged from 5 to 50 ng/ml (n=370), and apparently abnormal levels of CA‐III from 100 to 1900 ng/ml (n=27) were observed. The concentrations of immunoreactive CA‐III in the extracts of various equine tissues were also determined; it was present at high concentrations in skeletal muscle and liver and to a much lesser extent in the thymus. Other tissues contained much smaller amounts.