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Detection of early pregnancy in mares by the Rosette Inhibition Test and measurement of serum progesterone
Author(s) -
BRANCO M. D. L.,
KUCHEMBUCK M. R. G.,
PAPA F. O.,
FILHO E. P. CAMPOS
Publication year - 1989
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1989.tb04666.x
Subject(s) - estrous cycle , rosette (schizont appearance) , ovulation , pregnancy , andrology , early pregnancy factor , endocrinology , horse , medicine , biology , physiology , immunology , gestation , genetics , paleontology
Summary Early pregnancy factor (EPF) appears to be a protein complex that depends on pregnancy. It has been detected in the serum of several animal species using the Rosette Inhibition Test. In the present study 30 adult, Mangalarga mares with normal reproductive activity were divided into two groups: an experimental group which were mated and/or inseminated, and an unmated control group. In the experimental group, blood samples were taken on the 7th, 14th and 21st days after ovulation. In the control group, blood samples were collected from seven mares in oestrus and eight in dioestrus. In the control group the Rosette Inhibition Titre (RIT) ranged from 3 to 8 × 10 3 , whereas in the experimental group a RIT higher than 8 × 10 3 was considered an indication of the presence of EPF. Serum progesterone concentrations on Day 7 were equal to or greater than those measured in the mated mares on Day 14 or Day 21.