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Characterisation of IgE‐mediated histamine release from equine basophils in vitro
Author(s) -
MAGRO A. M.,
RUDOFSKY U. H.,
SCHRADER W. P.,
PRENDERGAST J.
Publication year - 1988
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1988.tb01544.x
Subject(s) - histamine , in vitro , immunoglobulin e , chemistry , calcium , basophil , liberation , pharmacology , immunology , biochemistry , medicine , antibody , organic chemistry
Summary In vitro IgE‐mediated histamine release by equine blood basophils was characterised as the basis for a screening test for immediate hypersensitivity responses in horses. The responses are initiated by inducing agents that are capable of crosslinking or bridging the membrane‐bound IgE molecules. The release process is complete within 40 mins. In vitro histamine release is dose‐dependent, with a submaximal response at less or greater than the optimal dose of inducing agent. Exogenous calcium is required but not magnesium; the optimal release calcium concentration is 1.0 to 1.5 mM. If an IgE‐mediated inducing agent is added in the absence of exogenous calcium, the basophils become desensitised. The pH and temperature optima for release are physiological (pH 7.4, 37°C). Histamine release is potentiated by deuterium oxide.