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Application of cloned fragments of equine herpesvirus type‐1 DNA for detection of virus‐specific DNA in equine tissues
Author(s) -
MORRIS CLAIRE M.,
FIELD H. J.
Publication year - 1988
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1988.tb01541.x
Subject(s) - virology , virus , dot blot , biology , dna , western blot , equine herpesvirus 1 , virus isolation , pathogenesis , microbiology and biotechnology , herpesviridae , viral disease , gene , immunology , genetics
Summary Tissue specimens obtained from equine herpesvirus‐1 (EHV‐1), subtype 1‐infected aborted foetuses were analysed for the presence of virus DNA by means of Southern blot and dot blot hybridisations. The specificity of the methods was confirmed although the sensitivity was inferior to classical techniques such as virus isolation. However, the possibility of detecting the state of the virus DNA and the ability to distinguish between subtypes were important features, and the dot blot method was shown to have potential for a rapid diagnostic test. This report demonstrates some potential practical applications of hybridisation methods for studying the pathogenesis and epidemiology of EHV‐1 but also reveals limitations of the techniques.