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Immunocytochemical localisation of carbonic anhydrase isozyme III in equine skeletal muscle
Author(s) -
NISHITA T.,
MATSUSHITA H.,
KAI M.
Publication year - 1987
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/j.2042-3306.1987.tb02660.x
Subject(s) - isozyme , carbonic anhydrase , skeletal muscle , chemistry , oxidative phosphorylation , horse , staining , biochemistry , glycolysis , succinate dehydrogenase , ouchterlony double immunodiffusion , enzyme , microbiology and biotechnology , biology , anatomy , antibody , antiserum , paleontology , genetics , immunology
Summary The location of carbonic anhydrase III (CA‐III) in frozen sections of biopsies of Thoroughbred horse skeletal muscle was studied. Fibre types were determined by ATP‐ase and succinate dehydrogenase staining. CA‐III isozyme was detected using a peroxidase conjugated anti‐CA‐III antibody. CA‐III was found to be localised in slow twitch oxidative fibres (ST), but was also present in fast twitch oxidative (FTH) fibres in small amounts. Fast twitch glycolytic (FT) fibres were stained lightly compared with control sections. The concentrations of CA‐III in muscle and liver were 70 μg/mg protein and 4 μg/mg protein, respectively. CA‐I and CA‐II were not found in muscle extracts by the double immunodiffusion method.