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Characterization of a new serotype g isolate of Aggregatibacter actinomycetemcomitans
Author(s) -
Takada K.,
Saito M.,
Tsuzukibashi O.,
Kawashima Y.,
Ishida S.,
Hirasawa M.
Publication year - 2010
Publication title -
molecular oral microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 77
eISSN - 2041-1014
pISSN - 2041-1006
DOI - 10.1111/j.2041-1014.2010.00572.x
Subject(s) - aggregatibacter actinomycetemcomitans , serotype , microbiology and biotechnology , chemistry , polysaccharide , strain (injury) , rhamnose , antigen , biology , bacteria , porphyromonas gingivalis , biochemistry , genetics , anatomy
Summary Aggregatibacter actinomycetemcomitans is usually isolated from the oral cavity where it is associated with active periodontitis. The species can be divided into six serotypes (a–f) according to their surface carbohydrate antigens. However, some clinical isolates cannot be grouped within these six serotypes. Gram‐negative, facultative anaerobic, catalase‐positive coccobacilli were isolated from a patient with periodontitis and identified by employing genetic, biochemical and serological analyses. Phenotypic data identified the isolate as A. actinomycetemcomitans. Serotype‐specific polysaccharide antigen from the isolate was untypeable by immunodiffusion testing in comparison with reference A. actinomycetemcomitans serotype a to f strains. Biofilm formation by the isolate was strong but cytotoxic activity was low. Gas chromatography/mass spectroscopy analysis of partially methylated alditol acetates from surface polysaccharide showed the presence of 2,4‐di‐ O ‐methyl‐rhamnose and 2,3,6‐tri‐ O ‐methyl‐glucose, with a 1 : 1 m ratio. The 1 H‐ and 13 C‐nuclear magnetic resonance spectra of the antigen showed that both constituent glycoses had α‐anomeric configuration. It is proposed that the untyped strain is a new A. actinomycetemcomitans serotype, designated serotype g.