Open AccessUrinary Leukotriene E4 Concentrations as a Potential Marker of Inflammation in Dogs with Inflammatory Bowel Disease
Author(s) -
Im Hof M.,
Schnyder M.,
Hartnack S.,
StankeLabesque F.,
Luckschander N.,
Burgener I.A.
Publication year - 2012
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/j.1939-1676.2011.00867.x
Subject(s) - medicine , creatinine , urinary system , leukotriene e4 , inflammatory bowel disease , urine , gastroenterology , diarrhea , ulcerative colitis , percentile , urology , leukotriene , disease , asthma , statistics , mathematics
Background Inflammatory bowel disease ( IBD ) and food‐responsive diarrhea ( FRD ) are chronic enteropathies of dogs ( CCE ) that currently can only be differentiated by their response to treatment after exclusion of other diseases. In humans, increased urinary concentrations of leukotriene E4 ( LTE 4) have been associated with active IBD . Objectives To evaluate urinary LTE 4 concentrations in dogs with IBD , FRD , and healthy controls, and to assess correlation of urinary LTE 4 concentrations with the canine IBD activity index ( CIBDAI ) scores. Animals Eighteen dogs with IBD , 19 dogs with FRD , and 23 healthy control dogs. Methods In this prospective study, urine was collected and CIBDAI scores were calculated in client‐owned dogs with IBD and those with FRD . Quantification of LTE 4 in urine was performed by liquid chromatography‐tandem mass spectrometry and corrected to creatinine. Results Urinary LTE 4 concentrations were highest in dogs with IBD (median 85.2 pg/mg creatinine [10th–90th percentiles 10.9–372.6]) followed by those with FRD (median 31.2 pg/mg creatinine [10th–90th percentiles 6.2–114.5]) and control dogs (median 21.1 pg/mg creatinine [10th–90th percentiles 9.1–86.5]). Urinary LTE 4 concentrations were higher in dogs with IBD than in control dogs ( P = .011), but no significant difference between IBD and FRD was found. No correlation was found between urinary LTE 4 concentrations and CIBDAI . Conclusions and Clinical Importance The higher urinary LTE 4 concentrations in dogs with IBD suggest that cysteinyl leukotriene pathway activation might be a component of the inflammatory process in canine IBD . Furthermore, urinary LTE 4 concentrations are of potential use as a marker of inflammation in dogs with CCE .