Open AccessConcentrations of Plasma Metabolites, Hormones, and mRNA Abundance of Adipose Leptin and Hormone‐Sensitive Lipase in Ketotic and Nonketotic Dairy Cows
Author(s) -
Xia C.,
Wang Z.,
Xu C.,
Zhang H.Y.
Publication year - 2012
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/j.1939-1676.2011.00863.x
Subject(s) - leptin , medicine , endocrinology , ketosis , insulin , hormone , adipose tissue , lipase , obesity , biology , diabetes mellitus , enzyme , biochemistry
Background Ketosis is an important metabolic disorder of dairy cows during the transition period. There have been many reports on the etiology of ketosis in periparturient cows, but little is known about its molecular etiology. Objectives The objective of this study was to clarify the status of fat mobilization and mRNA abundance of leptin and hormone‐sensitive lipase in cows with spontaneous clinical ketosis. Animals Ten ketotic H olstein cows and 10 nonketotic H olstein cows were used as the experimental animals. Methods Six blood biochemical parameters were evaluated by means of individual analysis method for 2 groups of cows. The mRNA abundance of leptin and hormone‐sensitive lipase in tail fat tissue from 2 groups of cows was measured by real‐time ( RT )‐ PCR , with a fluorescent T aqman probe and a standard curve. Results The plasma concentrations of glucose ( P = 0.01), and leptin ( P = 0.03), insulin ( P = 0.05), and the ratio of insulin to glucagon ( P = 0.04) were lower in ketotic compared with nonketotic cows, whereas there were marked increases in the plasma concentrations of nonesterified fatty acid and β‐hydroxybutyric acid ( P = 0.005). The mRNA abundance of leptin ( P = 0.04) and hormone‐sensitive lipase ( P = 0.02) in the fat tissue of ketotic cows was lower relative to that of nonketotic cows. Conclusions and Clinical Importance The ketotic cows showed characteristics of type I ketosis and some adaptive changes to negative energy balance in the plasma leptin concentration and mRNA abundance of fat leptin and hormone‐sensitive lipase.