Open AccessAntimicrobial Resistance Profiles and Clonal Relatedness of Canine and Feline Escherichia coli Pathogens Expressing Multidrug Resistance in the United States
Author(s) -
Shaheen B.W.,
Boothe D.M.,
Oyarzabal O.A.,
Smaha T.
Publication year - 2010
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/j.1939-1676.2009.0468.x
Subject(s) - pulsed field gel electrophoresis , cefpodoxime , enrofloxacin , amoxicillin , microbiology and biotechnology , multiple drug resistance , antibiotic resistance , escherichia coli , biology , drug resistance , phenotype , antimicrobial , antibiotics , ciprofloxacin , medicine , genotype , antibacterial agent , genetics , gene
Background: Antimicrobial resistance is increasing among Escherichia coli isolates associated with spontaneous infection in dogs and cats. Objectives: To describe E. coli resistance phenotypes and clonal relatedness and their regional prevalence. Animals: Isolates of E. coli (n = 376) collected from dogs and cats in the United States between May and September 2005. Methods: Isolates submitted from the South, West, Northeast, and Midwest regions of the United States were prospectively studied. Phenotype was based on E‐test susceptibility to 7 antimicrobials. Isolates were classified as no (NDR), single (SDR), or multidrug resistance (MDR). Clonal relatedness was determined by pulsed‐field gel electrophoresis (PFGE). Results: One hundred and ninety‐three (51%) isolates expressed resistance to at least 1 drug, yielding 42 phenotypes. SDR isolates (n = 84; 44%, 8 phenotypes), expressed resistance most commonly to amoxicillin (30%, n = 25) and least commonly to cefpodoxime (1%, n = 1). MDR isolates (n = 109; 56%, 31 phenotypes) were resistant to amoxicillin (96%, n = 105), amoxicillin‐clavulanate (85%, n = 93), and enrofloxacin (64%, n = 70); 18% (n = 20) were resistant to all drugs tested. The frequency of MDR did not differ regionally ( P = .066). MDR minimum inhibitory concentrations (MICs) were 6‐fold higher than SDR MICs ( P < .0001). Dendrograms of 91 isolates representing 25 phenotypes revealed 62 different PFGE profiles. Conclusions and Clinical Importance:E. coli strains spontaneously infecting dogs and cats are genetically and phenotypically diverse. Given the current prevalence of MDR among clinical isolates of E. coli in United States, implementation of a robust surveillance program is warranted.