Open AccessEffects of Time of Collection and Sample Processing on von Willebrand Factor Concentration in Retired Racing Greyhounds
Author(s) -
Marín L.M.,
Couto C.G.,
Iazbik M.C.,
Westendorf N.,
Vilar Saavedra P.
Publication year - 2008
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/j.1939-1676.2008.0198.x
Subject(s) - medicine , von willebrand factor , zoology , andrology , platelet , biology
Background: Concentrations of von Willebrand factor (vWF) are less than 30% in approximately 10% of Greyhounds. Hypothesis: That sample collection, processing, and storage can affect the concentration of vWF in plasma of Greyhounds. Animals: Nineteen healthy former racing Greyhounds. Methods:Prospective study : Blood samples were obtained from the jugular vein of dogs at 4 times during the day. Samples were divided and processed in each of 3 ways. The 1st tube was centrifuged immediately, the 2nd tube was kept in a household refrigerator, and the 3rd tube was kept at room temperature for 3 hours before centrifugation and processing. Results: There were no significant differences in the vWF concentration between different sample storage and processing ( P =5.31). There was no statistically significant diurnal variation in vWF concentration in the samples evaluated ( P =.37). Conclusions and Clinical Importance: Time of sample collection and short‐term storage temperature do not influence the vWF concentration in retired racing Greyhounds.