Open AccessPlasma Asymmetric Dimethylarginine, Symmetric Dimethylarginine, l ‐Arginine, and Nitrite/Nitrate Concentrations in Cats with Chronic Kidney Disease and Hypertension
Author(s) -
Jepson R.E.,
Syme H.M.,
Vallance C.,
Elliott J.
Publication year - 2008
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/j.1939-1676.2008.0075.x
Subject(s) - medicine , asymmetric dimethylarginine , endocrinology , cats , endothelial dysfunction , nitric oxide , creatinine , renal function , proteinuria , blood pressure , kidney disease , kidney , arginine , chemistry , biochemistry , amino acid
Background: Chronic kidney disease (CKD) and hypertension have been associated with decreased bioavailability of nitric oxide (NO) and endothelial dysfunction. Increased concentrations of the endothelial nitric oxide synthase (eNOS) inhibitor asymmetric dimethylarginine (ADMA) are implicated. Hypothesis: Plasma ADMA concentration is increased in cats with CKD and systemic hypertension corresponding to a decrease in total plasma nitrate/nitrite (NO x ) availability. Decrease in systolic blood pressure (SBP) and proteinuria during treatment of hypertension with amlodipine besylate may be associated with increased NO x availability. Animals: Sixty‐nine client‐owned normotensive and hypertensive cats with variable azotemia. Methods: Plasma ADMA, symmetric dimethylarginine (SDMA), and l ‐arginine were measured simultaneously by hydrophilic‐interaction liquid chromatography‐electrospray tandem mass spectrometry in cats from 6 groups: normotensive nonazotemic (n = 10), normotensive mildly azotemic (n = 10), hypertensive mildly azotemic with hypertensive retinopathy (n = 20), hypertensive mildly azotemic without hypertensive retinopathy (n = 10), normotensive moderately azotemic cats (n = 10), and hypertensive nonazotemic cats (n = 9). Plasma NO x concentrations were measured. Results: A moderate correlation between plasma creatinine and ADMA (n = 69, r = .608, P < .001), SDMA (n = 69, r = .741, P < .001), and NO x concentrations (n = 69, r = .589, P < .001) was observed. There was no association among plasma ADMA, SDMA, and NO x concentrations and SBP. Conclusions and Clinical Importance: Plasma ADMA and SDMA concentrations are increased in cats with CKD and correlate with plasma creatinine concentration. This may imply the presence of endothelial dysfunction in cats with CKD. Plasma ADMA concentrations were not associated with systemic hypertension. Treatment of systemic hypertension with amlodipine besylate did not affect plasma ADMA or NO x concentrations.