Open AccessEffects of 2 Concentrations of Sodium Citrate on Coagulation Test Results, von Willebrand Factor Concentration, and Platelet Function in Dogs
Author(s) -
Morales F.,
Couto C.G.,
Iazbik M.C.
Publication year - 2007
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/j.1939-1676.2007.tb02992.x
Subject(s) - sodium citrate , platelet , partial thromboplastin time , medicine , prothrombin time , von willebrand factor , hematocrit , sodium , hemostasis , coagulation , fibrinogen , platelet poor plasma , endocrinology , chemistry , platelet rich plasma , pathology , organic chemistry
Background:Blood collection tubes containing 3.2% (0.109 M) sodium citrate, instead of 3.8% (0.129 M) sodium citrate, have recently become available in the United States. These tubes are visually indistinguishable from the traditional 3.8% sodium citrate tubes, except for wording on the label. Consequently, samples for hemostatic evaluation are frequently collected in tubes containing the lower concentration of sodium citrate. Hypothesis:Results of hemostasis assays are different in samples collected in 3.2% versus 3.8% sodium citrate. Animals:Twenty healthy dogs. Methods:This study aimed at determining whether results of standard coagulation tests, von Willebrand factor concentration (vWF:Ag), and platelet function with the platelet function analyzer PFA‐100 a were affected by the different concentrations of sodium citrate. Blood samples were collected in tubes containing either 3.2% or 3.8% sodium citrate concentrations and processed routinely for coagulation assays (one‐stage prothrombin time [OSPT], activated partial thromboplastin time [aPTT], fibrinogen concentration, and platelet count), vWF:Ag, and platelet function assays with a PFA‐100. Results:There was no significant difference between samples collected in 3.2% versus those collected in 3.8% sodium citrate for OSPT, aPTT, fibrinogen concentration, platelet count, or vWF:Ag. The closure times with collagen/adenosine diphosphate were significantly shorter (66 ± 8.1 versus 74.8 ± 9.7 seconds; P < .0001) with the 3.2% than with 3.8% sodium citrate concentration, and the hematocrit was significantly higher (47.9 ± 5.6 versus 46.0 ± 4.7 seconds; P = .03) in samples collected in 3.2% than in those collected in 3.8% sodium citrate. Conclusions and Clinical Importance: There is no clinically relevant effect of collection of blood into 3.2% or 3.8% sodium citrate.