Open AccessEstimation of Quantitative Enzymuria in Dogs With Gentamicin‐Induced Nephrotoxicosis Using Urine Enzyme/Creatinine Ratios From Spot Urine Samples
Author(s) -
Grauer Gregory F.,
Greco Deborah S.,
Behrend Ellen N.,
Mani Indu,
Fettman Martin J.,
Allen Timothy A.
Publication year - 1995
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/j.1939-1676.1995.tb01091.x
Subject(s) - urine , creatinine , excretion , medicine , urinary system , renal function , endocrinology , gentamicin , chemistry , biochemistry , antibiotics
The correlation between 24‐hour urinary excretion of N ‐acetyl‐β‐ d ‐glucosaminidase (NAG) and γ‐glutamyl transferase (GGT) with urine NAG and GGT/creatinine ratios was assessed in dogs with gentamicin‐induced nephrotoxicosis. Eighteen 6‐month‐oid male Beagles with normal renal function were randomly divided into 3 groups of 6. Each group was fed a different concentration of protein (high protein, 27.3%; medium protein, 13.7%; and low protein, 9.4%) for 21 days. After dietary conditioning, gentamicin was administered at a dose of 10 mg/kg IM tid for 8 days and each group was continued on its respective diet. Endogenous creatinine clearance and 24‐hour urinary excretion of NAG and GGT were determined after dietary conditioning (day 0) and on days 2, 4, 6, and 8 of gentamicin administration. In addition, urine NAG and GGT/creatinine ratios (IU/L ± mg/dL) were determined from catheterized spot urine samples obtained between 7 and 10 am on the same days. The correlation between 24‐hour urinary enzyme excretion and urine enzyme/creatinine ratio in the spot urine samples was evaluated by simple linear regression analysis. Spot sample urine enzyme/creatinine ratios were significantly correlated with 24‐hour urinary enzyme excretion through day 4 for dogs on low dietary protein, through day 6 for those on medium protein, and through day 8 for those on high dietary protein. Mean ± SD baseline values for urine NAG/creatinine ratio and 24‐hour urinary NAG excretion were 0.06 ± 0.04 and 0.19 ± 0.14 IU/kg/24 hr, respectively. Baseline values for urine GGT/creatinine ratio and 24‐hour urinary GGT excretion were 0.39 ± 0.18 and 1.42 ± 0.82 IU/kg/24 hr, respectively.