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Detection of alkaline phosphatase in canine cells previously stained with W right– G iemsa and its utility in differentiating osteosarcoma from other mesenchymal tumors
Author(s) -
Ryseff Julia K.,
Bohn Andrea A.
Publication year - 2012
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2012.00445.x
Subject(s) - osteosarcoma , pathology , alkaline phosphatase , sarcoma , immunohistochemistry , stain , staining , giemsa stain , medicine , microbiology and biotechnology , chemistry , biology , enzyme , biochemistry
Background Osteosarcoma ( OSA ) is a common primary bone tumor in dogs. Demonstration of alkaline phosphatase ( ALP ) reactivity by tumor cells on unstained slides is useful in differentiating osteosarcoma from other types of sarcoma. However, unstained slides are not always available. Objectives The objectives of this study were to evaluate the diagnostic utility of detecting ALP expression in differentiating osteosarcoma from other sarcomas in dogs using cytologic material previously stained with W right– G iemsa stain and to assess the sensitivity and specificity of ALP expression for diagnosing osteosarcoma using a specific protocol. Methods Archived aspirates of histologically confirmed sarcomas in dogs that had been previously stained with W right– G iemsa stain were treated with 5‐bromo, 4‐chloro, 3‐indolyl phosphate/nitroblue tetrazolium ( BCIP / NBT ) as a substrate for ALP . Cells were evaluated for expression of ALP after incubation with BCIP / NBT for 1 hour. Sensitivity and specificity of ALP expression for diagnosis of OSA were calculated. Results In samples from 83 dogs, cells from 15/17 OSA s and from 4/66 tumors other than OSA (amelanotic melanoma, gastrointestinal stromal tumor, collision tumor, and anaplastic sarcoma) expressed ALP . Sensitivity and specificity of ALP expression detected using BCIP / NBT substrate applied to cells previously stained with W right– G iemsa stain for OSA were 88 and 94%, respectively. Conclusions ALP expression detected using BCIP / NBT substrate applied to previously stained cells is useful in differentiating canine OSA from other mesenchymal neoplasms.