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Cytologic features of clear cell adnexal carcinoma in 3 dogs
Author(s) -
Piviani Martina,
Sánchez Melissa D.,
Patel Reema T.
Publication year - 2012
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2012.00434.x
Subject(s) - pathology , pleomorphism (cytology) , cytopathology , carcinoma , differential diagnosis , clear cell , clear cell carcinoma , medicine , fine needle aspiration , biopsy , biology , cytology , immunohistochemistry
Background Clear cell adnexal carcinoma is a rare cutaneous adnexal neoplasm without definitive apocrine, sebaceous, or follicular differentiation. Differential diagnoses include sebaceous carcinoma, liposarcoma, and balloon cell melanoma. Immunohistochemical analysis, with positive immunoreactivity for pancytokeratin and vimentin, aids in the diagnosis. Cytologic features of clear cell adnexal carcinoma have not been described previously. Objective The aim of this study was to describe cytologic features of canine clear cell adnexal carcinoma. Methods Fine‐needle aspirates ( FNA ) obtained prior to biopsy of cutaneous neoplasms with a histologic diagnosis of clear cell adnexal carcinoma confirmed by immunohistochemical analysis were reviewed retrospectively. Slides prepared from FNA had been stained with modified W right‐ G iemsa or automated aqueous R omanowsky stain. Results Of 20 neoplasms diagnosed as clear cell adnexal carcinoma in dogs, FNA of the mass had been performed in 3 cases. Cytologic features were similar and included high cellularity, marked cellular pleomorphism, loose arrangement of neoplastic cells, and a light blue to gray background resulting from streaming of cytoplasm from ruptured cells. Neoplastic cells were oval to polygonal to spindle‐shaped with wispy cytoplasmic projections. Cytoplasmic eosinophilic stippling, globular deposits, or pink needle‐shaped inclusions were noted. Criteria of malignancy included marked anisocytosis, anisokaryosis and anisonucleoleosis, multinucleation, karyomegaly, macronucleoli, and atypical mitotic figures. Conclusions Clear cell adnexal carcinoma should be included in the cytologic differential diagnosis for a canine cutaneous mass even if an epithelial origin is not readily identified owing to lack of characteristic epithelial features, such as highly cohesive cell clusters, evident cell‐to‐cell junctions, and distinct cytoplasmic edges.