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Evaluation of thrombelastographic platelet‐mapping in healthy cats
Author(s) -
Blois Shauna L.,
Banerjee Amrita,
Darren Wood R.
Publication year - 2012
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2012.00419.x
Subject(s) - fibrin , platelet , thrombelastography , thrombin , adenosine diphosphate , chemistry , cats , thromboelastography , coefficient of variation , arachidonic acid , whole blood , medicine , endocrinology , biochemistry , immunology , enzyme , chromatography , platelet aggregation
Background Thrombelastography ( TEG ) permits analysis of clot formation but it is not specific for platelet activity. TEG P latelet M apping ( TEG ‐ PM ) is a modification of TEG that uses adenosine diphosphate ( ADP ) and arachidonic acid ( AA ) as platelet agonists to define the contribution of platelets to clot formation. Objectives The objectives of this study were to determine values for TEG ‐ PM in healthy cats and the interassay variation of TEG ‐ PM . Methods TEG ‐ PM analysis was performed on blood specimens collected from 12 healthy cats and was repeated using a second blood specimen collected 2 hours later. Maximum amplitudes generated by thrombin ( MA thrombin ), fibrin ( MA fibrin ), ADP ‐stimulated platelet activity ( MA ADP ), and AA ‐stimulated platelet activity ( MA AA ) were recorded. Results Mean ± SD for MA thrombin was 51.1 ± 8.5 mm, for MA fibrin was 32.3 ± 17.7 mm, for MA ADP was 32.3 ± 15.0 mm, and for MA AA was 24.5 ± 12.2 mm. Mean MA ADP and MA fibrin were not significantly different, whereas mean MA AA was significantly lower than mean MA fibrin . Results from the first and second specimens were not significantly different. Correlation between the first and second specimens was moderate for MA thrombin , MA fibrin , and MA ADP , but was poor for MA AA . A high degree of variability (coefficient of variation 47.7–60.0%) was observed for MA fibrin , MA ADP , and MA AA . Conclusions As MA ADP and MA AA AA were the same as or lower than MA fibrin , a valid baseline to determine platelet‐stimulated clot formation could not be established. Considerable interassay variation and wide intervals for MA fibrin , MA ADP , and MA AA values in this study indicate that TEG ‐ PM should be used cautiously in feline patients. Several preanalytical factors should be examined in further detail.