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Post‐transfusion survival of biotin‐labeled allogeneic RBC s in adult horses
Author(s) -
Mudge Margaret C.,
Walker Naomi J.,
Borjesson Dori L.,
Librach Fred,
Johns Jennifer L.,
Owens Sean D.
Publication year - 2012
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2011.00384.x
Subject(s) - biotinylation , medicine , blood transfusion , streptavidin , biotin , flow cytometry , transfusion therapy , red blood cell , andrology , gastroenterology , immunology , biology , microbiology and biotechnology , biochemistry
Background Post‐transfusion survival of allogeneic RBC s has been reported to be much shorter in horses than in other species. We hypothesized that post‐transfusion survival of biotinylated allogeneic equine RBC s would be greater than the survival previously reported from studies using radioactive RBC ‐labeling techniques. Objective The study objective was to determine post‐transfusion survival of N ‐hydroxysuccinimide ( NHS )‐biotin‐labeled allogeneic equine RBC s transfused into adult horses. Methods Horses were adults and included 5 donors and 5 recipients. All horses were blood‐typed, and donors were paired with recipients based upon blood type and crossmatch results. Donor blood was collected in a volume of 4 L into citrate phosphate dextrose adenine‐1 and stored for 24 hours, labeled with NHS ‐biotin, and re‐infused into recipients. Post‐transfusion blood samples were collected at 15 minutes and at 1, 2, 3, 5, 7, 14, 21, 28, and 35 days. Biotin‐labeled RBC s were detected by flow cytometry using streptavidin–phycoerythrin. Post‐transfusion survival at 24 hours, lifespan, and half‐life of biotinylated RBC s were determined. Results Mean ± SD survival of biotinylated RBC s at 24 hours post‐transfusion was 95 ± 24%; the mean lifespan of transfused allogeneic RBC s was 39 days based on calculation of a linear regression survival curve, and mean post‐transfusion RBC half‐life was 20 days. Conclusions Post‐transfusion survival of 24‐hour stored equine allogeneic RBC s was greater than previously reported but less than that observed for other companion animal species. Mechanisms for the relatively short post‐transfusion lifespan of allogeneic equine RBC s remain unknown and warrant further study.