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Granulomatous lymphadenitis caused by Talaromyces helicus in a L abrador R etriever
Author(s) -
Tomlinson Julie Kristy,
Cooley Avery James,
Zhang Shuping,
Johnson Melanie Elaine
Publication year - 2011
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2011.00377.x
Subject(s) - basophilic , pathology , biology , hypha , grocott's methenamine silver stain , conidium , anatomy , microbiology and biotechnology , botany , medicine , staining
A 3‐year‐old spayed female L abrador R etriever was presented for right prescapular lymphadenomegaly. Examination of fine‐needle aspirates and impression smears of the node revealed many short hyphal structures found within macrophages and extracellularly. Hyphae were approximately 3 μm in diameter, were irregularly septate with nonparallel walls, and had a small clear halo surrounding a partially stained basophilic internal structure. Hyphae were tapered on one end and had oval to pyriform swellings of 7–10 μm on the other, resulting in a bulbous appearance. Fungal elements stained positively with G omori methenamine silver and P eriodic acid‐ S chiff stains. The dog was euthanized at the owner's request, and necropsy revealed marked peripheral and visceral lymphadenomegaly. Histopathologic examination of lymph nodes confirmed granulomatous lymphadenitis with many fungal hyphae. Fungal culture yielded pure cultures of organisms that failed to produce ascospores or conidia precluding morphologic identification. PCR was performed using pan‐fungal primers, ITS ‐1 and ITS ‐2, to amplify the intergenic spacer regions of ribosomal RNA ; the PCR product was sequenced and a BLAST search of the G en B ank databases at NCBI revealed 100% identity of the organism with Talaromyces helicus , the sexual form of Penicillium helicum . Talaromyces helicus has not previously been reported to cause disease in people or animals.