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Processing of equine bone marrow using the automated M arrow X press System: RBC depletion, volume reduction, and mononuclear cell recovery
Author(s) -
Owens Sean D.,
Burges Julie,
Johns Jennifer L.,
Carrade Danielle D.,
Galuppo Larry D.,
Librach Fred,
Borjesson Dori L.
Publication year - 2011
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2011.00368.x
Subject(s) - peripheral blood mononuclear cell , reduction (mathematics) , volume (thermodynamics) , bone marrow , chemistry , biology , immunology , physics , biochemistry , in vitro , geometry , mathematics , quantum mechanics
Background The therapeutic use of bone marrow‐derived mononuclear cells ( MNC s) and mesenchymal stem cells for the treatment of soft tissue and orthopedic injuries in equine patients is expanding. After collection, bone marrow must be reduced in volume and depleted of RBC s for immediate therapeutic use or to prepare cells for culture or cryopreservation and storage. The M arrow X press ( MXP ) System is an automated, closed, sterile system designed to process human bone marrow samples. Objectives The purpose of this study was to evaluate the capacity of the MXP System to process equine bone marrow to reduce volume, deplete RBC s, and enhance recovery of MNC s. Methods Bone marrow was collected from 47 horses into 2 60‐ mL syringes containing heparin and processed using the MXP System. HCT , total nucleated cell ( TNC ) count, and MNC count were obtained for each sample before and after processing using an Advia 120 hematology analyzer. Volume reduction, RBC depletion, and recovery of TNC s and MNC s were calculated. Results For equine bone marrow samples, mean values were 73.2% for RBC depletion and 78.0% for volume reduction. TNC count before processing was 2.5 ± 1.2 × 10 7 and after processing was significantly higher at 7.8 ± 3.3 × 10 7 ( P < .0001), with a recovery of 68.5 ± 24.5% (mean ± SD ). MNC count before processing was 1.1 ± 0.9 × 10 7 and after processing was significantly higher at 3.8 ± 1.9 × 10 7 ( P < .0001), with a recovery 73.0 ± 31.5%. Conclusions The MXP System can reliably reduce volume and deplete RBC s from aspirates of equine bone marrow aspirates. MNC s can be recovered in a reproducible and sterile manner. Further studies evaluating the effects of the MXP System on cell viability, identification of mesenchymal stem cells ( MSC s), and the efficacy of MSC expansion are warranted.