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Dendritic cell leukemia in a Golden Retriever
Author(s) -
Allison Robin W.,
Brunker Jill D.,
Breshears Melanie A.,
Avery Anne C.,
Moore Peter F.,
Affolter Verena K.,
Vernau William
Publication year - 2008
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2008.00042.x
Subject(s) - pathology , histiocytic sarcoma , histiocyte , immunophenotyping , bone marrow , malignant histiocytosis , biology , leukemia , dendritic cell , myeloid , medicine , immunology , antigen
Abstract: An 8‐year‐old castrated male Golden Retriever was evaluated for decreased appetite, lethargy, and labored breathing of 1‐week duration. Bilateral pulmonary infiltrates, hepatomegaly, and splenomegaly were present. Results of a CBC revealed marked leukocytosis (62,600/μL; reference interval 4000–15,500/μL) and large numbers of atypical cells (30,700/μL) with abundant cytoplasm. There was no concurrent anemia, neutropenia, or thrombocytopenia. Morphology of the atypical cells was most consistent with a histiocytic origin. Similar cells were identified in bone marrow aspirates, and were morphologically suggestive of the macrophage variant of disseminated histiocytic sarcoma. However, flow cytometry of the abnormal circulating cells revealed CD1c, CD11c, and major histocompatibility complex (MHC) Class II expression without expression of CD11d or lymphoid markers, consistent with myeloid dendritic antigen‐presenting cells. At necropsy, the splenic architecture was effaced by neoplastic histiocytes that were also infiltrating lung, liver, an abdominal lymph node, myocardium, an bone marrow. Immunohistochemistry of the splenic neoplastic cells confirmed dendritic cell origin (CD1c+, CD11c+, MHC II+, no expression of CD11d and lymphoid markers). To the authors' knowledge, this is the first report of canine dendritic cell leukemia—in this instance accompanied by marked tissue infiltration.