Changes in gas composition and acid‐base values of venous blood samples stored under different conditions in 4 domestic species

Background:  The effect of storage temperature and time on blood gas and acid‐base values has been investigated intensively in cattle and dogs; however, data are lacking in other species. Objective : The aim of our study was to evaluate changes in gas composition and acid‐base values in venous blood stored at different temperatures and for different times in 4 domestic species in Italy.  Methods : Blood samples from Comisana sheep (n=10), Maltese goats (n=10), Ragusana donkeys (n=10), and Thoroughbred horses (n=10) were analyzed after storage at 23°C (room temperature) for 15 minutes (group I), 23°C for 1 hour (group II), 37°C for 8 hours (group III), and 4°C for 24 hours (group IV). Results were analyzed using a 1‐way repeated measures ANOVA.  Results : In all species no statistically significant differences in pH values were present in samples stored at 4°C for 24 hours. This also was true for Pco 2 in all species except the horse. Except for HCO 3 − concentration in the horse, significant changes in Po 2 , HCO 3 − concentration, base excess, and the standard bicarbonate concentration were observed for all species in samples stored at 4°C. In samples stored for only 1 hour at room temperature, significant changes in most analytes were detected.  Conclusions : The results of this study underline the need for rapid assessment of acid‐base samples, because any delay, even for 1 hour, may affect the results.