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Effect of repeated freeze‐thaw cycles on routine plasma biochemical constituents in canine plasma
Author(s) -
Reynolds Brice,
Taillade Benjamin,
Médaille Christine,
Palenché Florence,
Trumel Cathy,
Lefebvre Hervé Pierre
Publication year - 2006
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2006.tb00144.x
Subject(s) - chemistry , alkaline phosphatase , creatine kinase , centrifugation , creatinine , urea , chromatography , calcium , sodium , potassium , alanine aminotransferase , blood proteins , biochemistry , medicine , enzyme , organic chemistry
Background: The effect of repeated freeze‐thaw cycles on plasma constituents has not been assessed in dogs, although such a procedure is not uncommon to use in routine laboratory practice. Objective: To assess the effect of freeze‐thaw cycles on routine plasma constituents in healthy dogs. Methods: Six healthy adult dogs were used. Blood was sampled and placed in heparinized tubes. After centrifugation, plasma was separated into 5 aliquots. One aliquot was considered as the reference aliquot and used immediately for the assay of all of the biochemical constituents. All of the other aliquots were stored at −20°C. Three aliquots underwent 1, 2, or 3 freeze‐thaw cycles during a 1‐ to 3‐day period. The last aliquot remained at −20°C throughout the study and was thawed on the third day. The following biochemical constituents were assayed: glucose, urea, creatinine, total proteins, sodium, potassium, chloride, calcium, phosphates, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), and alkaline phosphatase (ALP). Results: No clinically relevant change was observed between the different aliquots for all of the constituents. Conclusion: Repeated freeze‐thaw cycles do not cause changes in the biochemical constituents studied in canine plasma.