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Creatinine concentration in plasma from dog, rat, and mouse: a comparison of 3 different methods
Author(s) -
Palm M.,
Lundblad A.
Publication year - 2005
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2005.tb00046.x
Subject(s) - creatinine , chromatography , high performance liquid chromatography , chemistry , picrate , protein precipitation , beagle , enzyme , medicine , biochemistry , ion , organic chemistry
Background: There are numerous methods for analyzing creatinine concentration in plasma, including the Jaffé alkaline picrate method in various modifications, enzymatic tests, and chromatographic methods. Objective: The purpose of this study was to evaluate whether an enzymatic method could replace a Jaffé method for routine creatinine measurements in plasma from dogs, rats, and mice. The enzymatic method and a compensated Jaffé method were tested against a high‐pressure liquid chromatography (HPLC) method, regarded as the gold standard for creatinine measurement. Methods: Heparinized plasma samples were obtained from 20 beagle dogs, 20 Wistar rats, and 20 CD1‐strain mice. The 2 test kits (Roche Diagnostics), Creatinine Jaffé Compensated and the enzymatic Creatinine Plus Version 2 reagent, were used on a Cobas Integra 400. The Jaffé compensated method used a calibration adjustment of −18 μmol/L to correct for the protein matrix in serum and plasma. The HPLC method was an isocratic method using a weak cation‐exchange column following protein precipitation. Results: Creatinine concentrations obtained using the enzymatic and the Jaffé methods differed significantly from the results obtained by the HPLC method. For dog plasma, mean values of 61.2, 61.8, and 67.8 μmol/L were obtained by the compensated Jaffé, enzymatic, and HPLC methods, respectively. In the rat, respective mean values were 26.7, 21.9, and 23.0 μmol/L, and in the mouse, respective mean values were 14.2, 5.4, and 9.2 μmol/L. Conclusion: The enzymatic method can replace the Jaffé method for plasma creatinine determination in dogs, rats, and mice because results from the enzymatic method were closer to HPLC values than were those of the Jaffé method.