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Differential leukocyte counts determined in chicken blood using the Cell‐Dyn 3500
Author(s) -
Lilliehöök Inger,
Wall Helena,
Tauson Ragnar,
Tvedten Harold
Publication year - 2004
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2004.tb00362.x
Subject(s) - eosinophil , hematology analyzer , blood smear , blood cell , granulocyte , hematology , monocyte , lymphocyte , cell counting , medicine , pathology , platelet , leukocyte counts , immunology , biology , cancer , asthma , malaria , cell cycle
Background: Automated hematology instruments commonly are used for mammalian blood analysis, but there is a lack of accurate automated methods available for avian leukocyte analysis. Objective: The aim of this study was to validate differential leukocyte counts in blood from chickens using the Cell‐Dyn 3500 hematology system and avian‐specific software. Methods: Blood samples were collected in lithium‐heparin tubes from 2 groups (n = 84 and n = 139) of laying hens. Manual 200‐cell differential counts were done on routinely‐stained blood smears, and manual total granulocyte counts (heterophils and eosinophils) were done using an eosinophil stain in a counting chamber. Automated differential counts were done using VET 2.3, a research and development version of avian‐specific software for the Cell‐Dyn 3500. Results were analyzed using Pearson's correlation and difference plots. Results: Automated granulocyte counts from the Cell‐Dyn were in good agreement with manual granulocyte counts ( r = 0.93 and 0.80 for the 2 study groups). No correlation was found between automated and manual lymphocyte counts. Correlation coefficients for monocyte counts were 0.70 and 0.43. Conclusion: Automated leukocyte results from the Cell‐Dyn using VET 2.3 software were not fully accurate. Total granulocyte counts may be of clinical usefulness, but results obtained for other parameters were unreliable.