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Validation of the Coulter AcT Diff Hematology Analyzer for Analysis of Blood of Common Domestic Animals
Author(s) -
Dawson Hilary,
Hoff Brent,
Grift Evert,
Tvedten Harold,
Shoukri Mohammed
Publication year - 2000
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.2000.tb00243.x
Subject(s) - hematology analyzer , autoanalyzer , hematology , medicine , cats , concordance , veterinary medicine , coulter counter , hemoglobin , biology , microbiology and biotechnology
The objective of this study was to compare and assess the agreement between the Coulter AcT Diff hematology analyzer (CAD) and the Bayer Technicon H1 (H1) using blood samples from 391 animals of 4 species. The H1 has been used in veterinary laboratories for many years. Recently, Coulter modified the CAD and added veterinary software for hematologic analysis of feline, canine, and equine samples. A comparison of hemograms from dogs, cats, horses, and cattle was made using EDTA‐anticoagulated blood samples. Both instruments were calibrated using human blood products. Performance characteristics were excellent for most values. The exceptions were MCV in canine samples (concordance correlation of .710), platelet counts for feline and equine samples (.258 and .740, respectively), feline and bovine WBC counts (.863 and .857, respectively), and bovine hemoglobin (.876).