Premium
Rapid Staining Techniques in Cytopathology: A Review and Comparison of Modified Protocols for Hematoxylin and Eosin, Papanicolaou and Romanowsky Stains
Author(s) -
Jörundsson Einar,
Lumsden John H.,
Jacobs Robert M.
Publication year - 1999
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1999.tb01057.x
Subject(s) - papanicolaou stain , staining , h&e stain , cytopathology , eosin , pathology , stain , medicine , fixation (population genetics) , cytology , cancer , population , cervical cancer , environmental health
— The objective of this study was to review and compare rapid protocols for fixation and staining of cytologic smears. We used fresh surgical specimens from dogs and horses to evaluate and modify, if necessary, previously described rapid staining protocols. Slides were wet‐fixed, rehydrated or air‐dried. Rapid Papanicolaou, hematoxylin and eosin (H&E), and Romanowsky stains were applied, including modifications of Diff‐Quik stain. The modified rapid staining protocols were simple to use and gave results within 5 minutes that were comparable to those obtained with traditional methods. Advantages of rehydrated vs wet‐fixed smears included consistent preparations, a clean background, and equally good or superior nuclear detail.