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Morphology, Cytochemical Staining and Ultrastructural Characteristics of Reindeer ( Rangifer tarandus ) Leukocytes
Author(s) -
Henkel Kurt A.,
Swenson Cheryl L.,
Richardson Burnell,
Common Ralph
Publication year - 1999
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1999.tb01035.x
Subject(s) - basophilic , staining , sudan black b , pathology , biology , basophil , microbiology and biotechnology , stain , cytochemistry , ultrastructure , chemistry , immunology , anatomy , medicine , antibody , immunoglobulin e
— Peripheral blood smears from four adult reindeer (Rangifer tarandus) were examined after staining with Romanowsky's stain and cytochemical stains, including α‐napthyl butyrate esterase (α‐NBE), Sudan black B (SBB), chloroacetate esterase (CAE) and alkaline phosphatase (ALP). Romanowsky‐stained eosinophils, neutrophils, lymphocytes and monocytes resembled those of cattle, sheep and goats. Basophils had two different staining patterns with Romanowsky's stain. Basophils that we termed“grey basophils”were similar in appearance to grey eosinophils in Greyhound dogs, with medium blue‐grey to lavender‐grey cytoplasm containing varying numbers of clear vacuoles or granules and variable numbers of small, intensely basophilic, perinuclear granules. The second basophil staining pattern was more typical of ruminant basophils, with uniform, pale to dark, basophilic cytoplasmic granules. Basophils stained positive for α‐NBE, SBB, CAE and ALP. Eosinophils stained positive for SBB, and were negative for α‐NBE, CAE and ALP. Neutrophils stained positive for SBB and CAE, and were negative for α‐NBE and ALP. Lymphocytes stained positive for α‐NBE, and were negative for SBB, CAE and ALP. Monocytes stained positive for α‐NBE, were rarely positive for CAE and SBB, and were negative for ALP. Transmission electron microscopy revealed matrix within all granulocyte granules, including those of basophils.