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Application of Rapid CD3 Immunophenotype Analysis and Argyrophilic Nucleolar Organizer Region (AgNOR) Frequency to Fine Needle Aspirate Specimens From Dogs With Lymphoma
Author(s) -
Vail David M.,
Kravis Laura D.,
Kisseberth William C.,
Ogilvie Gregory K.,
Volk Lynn M.
Publication year - 1997
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1997.tb00711.x
Subject(s) - immunophenotyping , nucleolus organizer region , pathology , lymphoma , biology , medicine , flow cytometry , nucleolus , microbiology and biotechnology , biochemistry , cytoplasm
Determinations of CD3 immunoreactivity (CD3‐IR) and argyrophilic nucleolar organizer region (AgNOR) frequency from fine needle aspirate (FNA) samples were compared with those from formalinfixed, paraffin‐embedded surgical biopsy samples in 51 dogs with lymphoma. Both CD3‐IR (using a rapid EPOS R polymer system) and AgNOR techniques were readily applied to FNA samples. CD3‐IR from FNA samples matched those of histologic samples with 100% concordance. A linear relationship (r = 0.981; P < 0.001>) was found between AgNOR frequency obtained from FNA samples and those obtained from surgical biopsy samples. Application of the techniques presented here should allow clinically relevant information to be procured rapidly and inexpensively. As CD3‐IR and AgNOR frequency have been shown to be predictive of response to combination chemotherapy in dogs with lymphoma, such information could be used to better educate clients as to the likelihood of achieving meaningful responses, as well as allowing prospective tailoring of individual treatments in future trials prior to initiating therapy.