Premium
Evaluation of Six Cytometric Methods for Reticulocyte Enumeration and Differentiationin the Cat
Author(s) -
Perkins Paula C.,
Grindem Carol B.
Publication year - 1995
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1995.tb00935.x
Subject(s) - acridine orange , reticulocyte , enumeration , orange (colour) , stain , nucleic acid , cytology , biology , staining , chromatography , microbiology and biotechnology , chemistry , biochemistry , food science , mathematics , genetics , rna , combinatorics , gene
Six protocols utilizing three fluorescent nucleic acid dyes (thiazole orange, auramine O, and acridine orange) were evaluated by flow cytometry for clinical utility in the analysis of feline reticulocytes. The dyes showed good to poor correlations with each other and with manual counts. Thiazole orange was the preferred stain. Thiazole orange dye showed distinct peaks corresponding to aggregate and punctate reticulocytes from specimens with marked reticulocytosis. The other dyes detected increases in reticulocyte numbers but subpopulations could not be distinguished. Standardized instrument and gate settings, applied to specimens stained with thiazole orange dye, enumerated aggregate and punctate reticulocytes in low cellularity specimens that lacked distinct peaks. Percentages of these cells correlated well with manual counts, offering a simplified technique for routine laboratory use.