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An Improved Ferritin Assay for Canine Sera
Author(s) -
Andrews Gordon A.,
Smith Joseph E.,
Gray Mary,
Chavey Patricia S.,
Weeks Bradley R.
Publication year - 1992
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1992.tb00584.x
Subject(s) - ferritin , serum ferritin , microbiology and biotechnology , monoclonal antibody , antibody , chemistry , medicine , pathology , immunology , biology
Summary An improved serum ferritin assay for canine serum has been developed. It uses two monoclonal antibodies in a sandwich arrangement. Serum ferritin can be determined on undiluted canine sera with this assay. The recovery of ferritin added to canine serum ranged from 98 to 106%, the within‐assay coefficient of variability was 3.3 to 4.5%, and the assay‐to‐assay variability was 9.8 to 10.2%. Serum ferritin from 61 apparently healthy dogs had a geometric mean of 252 ng/ml, with a range of 80 ng/ml to 800 ng/ml.