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Measurement of α‐Amylase Activity in Cat Plasma: Test of Specificity of a Chromogenic Substrate, Reference Values
Author(s) -
Ouedraogo G.,
Rico A.G.,
Braun J.P.
Publication year - 1992
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1992.tb00582.x
Subject(s) - chromogenic , hemolysis , cats , amylase , chemistry , substrate (aquarium) , reference values , alpha amylase , chromatography , medicine , endocrinology , biochemistry , enzyme , biology , ecology
Summary Glucoamylase activity can interfere with the measurement of α‐amylase activity in cat plasma. This can be avoided by the use of “blocked” substrates such as 4,6‐O‐benzylidene‐4‐nitrophenyl‐α‐D‐maltoheptaoside. In 241 healthy cats, reference values were 586 ± 241 U/L (m ± standard deviation) at 30°C; age, sex, overnight fasting, and mild hemolysis had no significant effect.