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Determination of Uric Acid in Canine Serum and Urine By High Performance Liquid Chromatography
Author(s) -
Felice Lawrence J.,
Dombrovskis Dzintra,
Lafond Elizabeth,
Bartges Joseph,
Osborne Carl A.
Publication year - 1990
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1990.tb00549.x
Subject(s) - urine , chromatography , uric acid , high performance liquid chromatography , chemistry , detection limit , phosphotungstic acid , biochemistry , catalysis
Summary A reproducible high performance liquid chromatography (HPLC) method was developed for analysis of uric acid in canine serum and urine. The method consists of precipitating serum proteins with phosphotungstic acid prior to HPLC analysis. Urine is analyzed after dilution with buffer. Chromatography is performed on a reversed‐phase C‐18 column with UV detection at 292 nm. Sensitivity of the method will allow reproducible measurement of uric acid at concentrations of 0.05 mg/dl in serum and 0.1 mg/dl in urine. The HPLC method has been used to quantify hundreds of canine serum and urine samples. The method is superior to UV absorption or colorimetric methods because its lower limit of detection allows measurement of uric acid at concentrations found in canine serum and urine.