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Utilization of an Enzyme Heat Stability Test and Erythrocyte Glycolytic Intermediate Assays to Assist in the Diagnosis of Canine Pyruvate Kinase Deficiency
Author(s) -
Harvey John W.,
Kociba Gary J.,
Peteya Donald J.
Publication year - 1990
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1990.tb00544.x
Subject(s) - pyruvate kinase , pyruvate kinase deficiency , glycolysis , heat stability , enzyme , biochemistry , chemistry , materials science , composite material
Summary A macrocytic hypochromic anemia, with marked reticulocytosis and large numbers of circulating nucleated erythrocytes, was recognized in a 3½‐year‐old male Beagle‐cross dog. A presumptive diagnosis of erythrocyte pyruvate kinase (PK) deficiency was made, even though PK activity from the patient was within the range of activities measured for normal dogs, because the PK activity should have been several times normal in light of the large number of reticulocytes present. The PK activity in a hemolysate from the patient was heat‐labile compared to activities in hemolysates from normal dogs, a finding consistent with results from a previous study of PK‐deficient Basenji dogs. Seven phosphorylated glycolytic intermediates and 2,3‐diphosphoglycerate were measured in protein‐free extracts of erythrocytes from the patient. All were present in concentrations above that present in normal canine erythrocytes, further supporting the diagnosis of PK deficiency.