Premium
Combined Phagocytosis‐Nitroblue Tetrazolium Reduction Test in Bovine Neutrophils
Author(s) -
Valinoti Jan M.,
Zinkl Joseph G.,
Jain Nemi C.
Publication year - 1988
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1988.tb00502.x
Subject(s) - zymosan , phagocytosis , formazan , nitroblue tetrazolium , phagosome , opsonin , chemistry , microbiology and biotechnology , biology , biochemistry , in vitro
Summary An assay was developed for the simultaneous evaluation of phagocytosis and oxidative metabolism of bovine blood neutrophils. Phagocytosis was evaluated by using opsonized zymosan, and oxidative metabolism was evaluated by nitroblue tetrazolium (NBT) reduction. Normal bovine neutrophils exhibited moderate variation in ability to phagocytize zymosan, but little variation in ability to reduce NBT. The subcellular location of NBT reduction to formazan was determined by electron microscopy. Electron dense formazan precipitate was observed along the inner membrane of phagosomes enclosing zymosan particles and radiating from the membrane toward the center of the phagosome.