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Serum Bile Acids: Reference Values in Healthy Dogs and Comparison of Two Kit Methods
Author(s) -
Counsell Linda J.,
Lumsden John H.
Publication year - 1988
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1988.tb00494.x
Subject(s) - postprandial , radioimmunoassay , serial dilution , medicine , gastroenterology , chromatography , reference range , chemistry , endocrinology , pathology , alternative medicine , insulin
Summary Serum bile acid (SBA) reference intervals were established by use of a radioimmunoassay method for fasting dogs to be 0.2 to 4.3 μmol/L (n = 60) and for 2 hour postprandial samples to be 0.6 to 24.2 μ mol/L (n = 37). The SBA reference intervals estimated using an enzymatic method were 0 to 8.6 μ mol/L for fasting (n = 26) and 0 to 29.8 μ mol/L for 2 hour postprandial samples (n = 36). The correlation between the two methods including samples from healthy dogs and clinical cases is good (n = 128, r = 0.82, p > 0.0001). The radioimmuno‐assay method is linear to 50 μ mol/L and the enzymatic method is linear to 100 μ mol/L, thus both methods require serum dilutions to be made in many cases of primary liver disease. The enzymatic method is less expensive and more convenient for use in a clinical laboratory but requires a greater sample volume (400 μl) than the RIA method (50 μl). Both methods have adequate precision and accuracy to be useful as diagnostic tests of liver function in dogs.