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A Technique for Automated Quantification of Canine Glucocorticoid‐Induced Isoenzyme of Alkaline Phosphatase
Author(s) -
Hoffmann Walter E.,
Sanecki Robin K.,
Dorner Joseph L.
Publication year - 1988
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1988.tb00493.x
Subject(s) - alkaline phosphatase , glucocorticoid , isozyme , chemistry , biology , biochemistry , endocrinology , enzyme
Summary A sensitive assay for the corticosteroid‐induced alkaline phosphatase isoenzyme (CAP), adaptable to most clinical chemistry autoanalyzers, is described and validated. This assay is based on the greater than 98% inhibition of liver alkaline phosphatase isoenzyme (LAP) activity with 4.2 mM levamisole, as compared to the 42% inhibition of CAP activity. Analysis of serum with total alkaline phosphatase (AP) activity within the reference range, resulted in a reference range of 0 to 19 U/L for CAP activity. Analysis of serum from 160 clinical patients with AP activity above the reference range, revealed 73% with increased CAP activity ranging from 20 to 7,000 U/L. The diagnostic significance of this increased CAP activity is discussed.